PGD (Pre-implantation Genetic Diagnostic)
PREIMPLANTATION GENETIC DIAGNOSIS
In recent years, developments in genetics allow genetic analysis to be carried out on embryos developed in vitro by in vitro fertilization ethods and the placement of selected healthy embryos in the uterus of the mother before pregnancy occurs. This method is called pre- regnancy genetic diagnosis (Preimplantation Genetic Diagnosis-PGD).
One of the most important application areas of the PGT technique embryos that do not have a genetic disease but may not have a genetic disorder in their chromosomes.
These disorders are more common in women of advanced age (35 years and older). This situation may result in infertility as it both reduces the chance of attachment of the embryo (implantation) and may cause unwanted abortions. As a matter of fact, 40 out of every 100 pregnancies in women aged 39 and over have problems that occur due to a numerical chromosomal disorder
The aim of PGD is to increase implantation rates, reduce spontaneous abortions and increase healthy baby birth rates by selecting hromosomally normal embryos. Indeed, the miscarriage rate, which can reach up to 23 percent in normal pregnancy due to chromosomal disorders, may decrease to 9 percent after PGD.
Pre-pregnancy genetic diagnosis is performed by taking 1 or 2 cells from embryos developed as a result of fertilization of egg and sperm cells obtained from mother and father candidates in laboratory environment. Special methods called Fluorescence In Situ Hybridization (FISH), Microarray-based comparative genomic hybridization (aCGH) or Polymerase Chain Reaction (PCR) are used for genetic diagnosis. Diagnosis of numerical chromosomal disorders such as monosomy or trisomy (Down syndrome and other trisomies) and single gene diseases (such as hemophilia, Mediterranean anemia, cystic fibrosis, muscular dystrophies) in the baby to be born is possible with PGD. Thus, healthy babies are born by transferring disease-free, healthy embryos to the expectant mother.
DIAGNOSIS BEFORE PREGNANCY
- In couples with a genetic or hereditary disease carrier,
- In couples who have a child or children with a previous genetic disease,
- For the purpose of HLA genotyping (tissue typing),
- In the identification of diseases showing genetic predisposition (tendency)
- Women in the advanced age group (37 years and over) who have been accepted for assisted reproductive techniques,
- In couples with recurrent early pregnancy miscarriages,
- In couples who could not achieve pregnancy with assisted reproductive techniques despite multiple applications or who lost their pregnancy due to miscarriage,
- It is applied in cases of chromosomal disorders or genetic diseases associated with severe male infertility.
HOW IS THE PGT TEST DONE?
- Whether the patient is suitable for PGD is evaluated by the reproductive health specialist, genetic counselor and the doctor of the related disease.
- The couple is prepared for the IVF procedure.
- The egg taken from the mother is fertilized in the laboratory with the sperm taken from the father.
- 1-2 blastomere cells are removed from the obtained embryo by biopsy by embryologists
- Cells obtained by biopsy are prepared and tested according to the method to be applied.
- Embryos with genetic diseases or chromosomal disorders are selected and discarded and healthy embryos are transferred to the mothers womb.
Microchip
In patients with low sperm viability, quantity, number and structural quality, microfluidic technology, also known as the "chip baby method", ensures that the best quality sperm cells are selected by competing with each other in a way similar to the natural environment in the uterus, and ICSI is defined in in vitro fertilization treatment. With the application, it is left into the egg cell by microinjection method.
In this method, sperm cells with the best structural form, genetics and physiology are selected without causing cell damage, since techniques such as centrifugation (settling), vortex (mixing with high vibration) or mixing with a pipette are not applied to the sperm cells, which can physically damage the sperm cells. In classical preparation methods, sperm cells are prepared in a shorter time without being subjected to manipulation by skipping processes such as washing, flotation, gradient (grading), centrifugation (precipitation at high speeds). Thus, the sperm cells with the best DNA quality and physiologically are selected by themselves, and the sperm cells that pass this pre-screening are again screened under the microscope and the sperm cells of excellent quality are used for microinjection.
The above-mentioned classical preparation methods cause sperm cells to undergo respiratory stress, known as oxidative stress, and it has been scientifically proven that this can also cause DNA damage in healthy sperm cells. Better quality embryos are tried to be obtained with sperm cells that have been proven to be of the best quality by scientific data, and thus embryos with the highest potential for implantation (attachment) and development are obtained. The average total mobility in suckling samples taken from male individuals with microchip technology increased by 1.7 times.
The sperm cells obtained by this method were found 3 times faster than the classical preparation methods, and the forward motile sperm cells increased 3.8 times.
Our Treatment Processes
DOCUMENTS TO BE REQUESTED FROM THE PATIENT
1. Biometric photo (.pdf)
• Digitally scanned biometric photos of the patient and companion
2. Flight ticket reservation (.jpeg or .pdf)
3. Hotel reservation (.jpeg or .pdf)
• In case the patient stays in the hospital during the treatment; The document prepared on the letterhead of the health institution stating that the accommodation service will be provided by the hospital will be accepted.
4. Proof of income (.jpeg or .pdf) showing that he can cover the treatment costs
• Bank-approved bank statement document containing the last 6 months. If the patient does not have his own bank account, his spouse will be accepted (an official document showing that he is a spouse will be attached)
5. Colored and legible scanned images (.jpeg or .pdf) of the front and back of the passport
6. Document showing the compulsory health or travel insurance of the patient
7. Receipt showing that the treatment fee has been paid to the health institution in Turkey
• At least $ 500 (USD) must be deposited into the hospital account, and the patient's name, surname and passport number must be written as an explanation.
Note: The documents must be requested from the patient with color scanning.
What Is insemination? How Is It Done?
There are 2 treatment methods most commonly preferred by couples who want to have a baby but do not have a baby for various reasons. One of them is in vitro fertilization treatment and the other is insemination method. In our article, we will try to answer the most frequently asked questions about the vaccination method, what is insemination and how it is done.
What is insemination?
If couples cannot get a pregnancy result after one year after unprotected sexual intercourse, infertility is diagnosed. Infertility can have many causes, depending on the woman and the man. Thanks to science and developing technology, there are now many treatment methods for infertility. One of these methods is the vaccination method. In its simplest form, the method of vaccination is to prepare the sperm in the laboratory and inoculate the woman's uterus at the time of ovulation to ensure fertilization. In order for the vaccination method to be performed, some tests must be done to make sure that there is no problem in the uterus of the expectant mother. In addition, at least one of the tubes of the expectant mother must be in working condition. The purpose of the vaccination method is to select the motile, normal sperm with a higher probability of fertilization and send them to the uterus of the expectant mother. Thus, it will be more likely to be fertilized with eggs and selected sperm supported by drugs or pills.
How is insemination done?
Before the insemination method, the development of the egg is supported with pills or low-dose injections given to the expectant mother. During this process, the egg is tracked. When the eggs mature and reach certain sizes, a cracking needle is given and the eggs are cracked. Just at this time of cracking, the sperm prepared in the laboratory are transferred to the uterus. Thus, the most suitable time of ovulation is provided for the woman and the best sperm are transferred to the uterus at the right time. insemination is a natural method. Only sperms that will increase the probability of fertilization are carefully selected. From the sperms taken from the father-to-be, those that are of good quality, motile and washed in a certain number, that is, processed. As Hüma IVF Center team, we tried to give information about what is insemination and how it is done in our article. We hope it was useful. You can contact us via our website to get more detailed information about IVF treatment or to ask your questions.
WHAT IS INTRALPID SERUM TREATMENT? WHO IS APPLIED?
WHAT IS INTRALPID SERUM TREATMENT? WHO IS APPLIED?
Intralipid serum therapy emerges as a form of treatment applied to women who want to become pregnant but cannot achieve this goal as a result of recurrent IVF failures. It is extremely important that the immune system is strong during IVF treatment. Intralipid serum therapy is a method used at this point to strengthen the immune system.
Intralipid serum treatment is started to be applied to women who want to become pregnant before starting IVF treatment. What are the details of intralipid treatment? Who is intralipid serum therapy applied to? How is intralipid serum therapy applied? Now we will be giving answers to all these questions for you below.
What is Intralipid Therapy?
Intralipid serum therapy is a treatment for women who want to become mothers. Intralipid serum is a treatment method that starts on the day of transfer and continues throughout the process in IVF treatment. Intralipid serum contains many important proteins and molecules for the immune system. Thanks to this serum, it is aimed to keep the immune system of the expectant mother strong during pregnancy by supporting the immune system.
To Whom Is Intralipid Serum Treatment Applied?
Intralipid serum therapy is a treatment for women who want to become mothers, but only for those who have experienced miscarriage problems or who have experienced IVF failures. The purpose of this treatment is to ensure that the immunity of the expectant mother remains strong throughout the pregnancy process.
As a result of the information obtained, it has been proven that intralipid serum therapy does not have any side effects. However, some women may be allergic to the substances in the intralipid serum. At this point, good research should be done before starting the treatment and if there is no allergic condition, treatment should be applied.
How is Intralipid Serum Treatment Applied?
Parents who want to have a child can resort to many ways for pregnancy to occur. In vitro fertilization, which has been very popular recently, has been determined as the most successful assisted reproductive method. If there are recurrent failures as a result of IVF treatment, this problem can be eliminated with intralipid serum treatment. Intralipid serum therapy is an extremely effective and successful form of treatment.
Intralipid serum treatment is administered to the expectant mother by intravenous route, with a preparation containing emulsified oil, prepared with a special concentration, which takes approximately 45 minutes. In other words, the patient's immune system is supported by giving oily serums intravenously.
In this article, we have given detailed explanations about inralipid serum therapy and that parents can receive intralipid serum treatment in cases where pregnancy does not occur due to some reasons. If you want to have more detailed information on this subject, you can visit https://www.humatupbebek.com/.
WHAT IS THE SPERMIOGRAM TEST FOR ANALYSIS OF SPERM CELL?
Parents who want to have children generally prefer the most common in vitro fertilization treatment. Before this treatment, both mother-to-be and father-to-be go through many tests. Father-to-be passed; The first of the tests to test for infertility or other problems is the sperm test, also known as the spermiogram test. What is the spermiogram test for the analysis of the sperm cell? How is spermiogram done? What do spermiogram test results mean? We will answer all these questions for you in detail below. We recommend that you continue reading our article to have detailed information about the sperm test.
What is a test (spermiogram)?
Sperm test done to men; It is a test to determine sperm health, sperm count, and whether the sperm are moving correctly in the channels. As a result of the sperm test, if the sperm shape is normal and there is a sufficient number of sperm, it is concluded that your reproductive ability is high. However, in some cases, men may have low sperm count or the ability to reproduce despite having abnormal semen. That's why doctors order a second test for the accuracy of the results. If your results are normal in your first sperm test, you give the other test and if it is normal, these two normal tests show that you do not have an infertility problem. If there are different conditions, your doctor may order other tests.
If there is no semen or sperm as a result of the sperm test, this may indicate that your ducts are blocked. If this is the case, your doctor will plan different surgeries to open the channels.
Issues and Precautions to Consider While Giving Sperm Sample
There are important points to be considered before giving the sperm sample and while giving the sperm sample. Among the things to be considered before giving a sperm sample, we can count that "men should abstain from sexual intercourse for 3 days". Fasting for 3 to 5 days is sufficient. These numbers are important because if you fast for less than 3 days, the sperm count may be low as a result of the sperm test. For this reason, we can say that fasting between 3 and 5 days is sufficient. It is possible to list the issues to be considered while giving a sperm sample as follows;
· Storing the sample in a sterile environment: It is important to protect your sample from the outside environment for a healthy and accurate spermiogram test result. If a sterile environment is not provided, the test result may be inaccurate. Therefore, do not put your hands, especially sample containers, etc. It is important that you keep it as sterile as possible.
· Protecting the delivered sperm from heat: Another important point is to keep your sample away from extreme temperature changes after ejaculation. Because these extreme temperature changes negatively affect spermatozoa. Ideal temperatures for the sample cup should be between 20°C and 37°C.
· Sperm volume, amount: Volume is extremely important in the sperm test. Your sperm volume is expected to be at least 1.5 ml. The reason why you cannot reach the lower limit of 1.5 ml may be external discharge during ejaculation. It is important to be careful about this.
· Use of lubricating foreign substances during the sperm test: It is important not to use any lubricant during the sperm test because these substances seriously affect the sperm parameters. External factors with such an impact can lead to wrong conclusions.
How is the Sperm Test Done?
For the sperm test, you must first be at the IVF center or hospital. There are rooms specially prepared for men in hospitals or IVF centers. In these rooms, men give their sperm in a special container. For the effectiveness of the sperm test, the sperm sample is immediately delivered to the laboratory. The sperm sample given in the laboratory is subjected to many processes and the best quality sperms are selected. The purpose of the procedures is to examine the sperm count, motility and shape in the semen and to reach quality sperm as a result.
Apart from the hospital, sperm samples can be placed in a container and brought to the hospital in the home environment. However, this is highly not recommended. Even if this is accepted, the sperm samples should be brought to the hospital and delivered within half an hour.
Issues and Precautions to Consider When Analyzing Sperm Sample
For the sperm test, you must first be at the IVF center or hospital. There are rooms specially prepared for men in hospitals or IVF centers. In these rooms, men give their sperm in a special container. For the effectiveness of the sperm test, the sperm sample is immediately delivered to the laboratory. The sperm sample given in the laboratory is subjected to many processes and the best quality sperms are selected. The purpose of the procedures is to examine the sperm count, motility and shape in the semen and to reach quality sperm as a result.
Apart from the hospital, sperm samples can be placed in a container and brought to the hospital in the home environment. However, this is highly not recommended. Even if this is accepted, the sperm samples should be brought to the hospital and delivered within half an hour.
Issues and Precautions to Consider When Analyzing Sperm Sample
After the sperm test is given by the prospective fathers, it is examined in the laboratory environment. This examination takes approximately 1-2 hours and after the first hour, active results begin to be obtained. However, sometimes different situations can be encountered. For example, the test is given, but no sperm can be seen when looked at. In these cases, sperm are taken again and centrifuged. When faced with such a situation, the time may be extended. If there is no additional test to be done, the test results are immediately available in an average of 1-1.5 hours.
What Do Sperm Test Results Mean?
Sperm test results can be evaluated in many ways. Sperm analysis evaluates the sperm by analyzing it from various aspects. We can list these analyzes as follows:
· Sperm count and density:
Sperm count describes the number of sperm in 1 ml of semen. The World Health Organization advocates that a normal sperm count should be at least 15 million per ml or at least 39 million per sample. Having fewer sperm than these numbers indicates a low sperm count.
· Sperm morphology:
Non-abnormal sperm have a long tail and oval head. Morphology refers to the size and shape of the sperm a person has. Sperm that do not have a normal size or are weak in shape have difficulty reaching the egg and fertilization.
· Sperm motility:
The ability of sperm to move efficiently is one of the most important factors for fertilization.
· PH level of sperm:
When the pH level is too high or too low, it can affect the health of sperm and its ability to pass through the female reproductive tract.
Apart from these, sperm health is examined by looking at many factors such as semen volume, liquefaction, and seminal fructose levels while performing the sperm test. After examining all factors in detail, the results are examined and reported to the patient.
What is the Sperm Test Result of a Healthy Individual?
Low sperm count can have many different causes. The first reason is smoking addiction. Smoking addiction is one of the important factors that reduce the sperm count. In addition to smoking, alcohol and drug addiction also affect sperm count. Sperm count can also be adversely affected when there is a deficiency in hormones, depending on the clogged ducts.
Hormones are one of the important factors for reproduction on their own. Since the deficiency in reproductive hormones directly affects sperm production, it increases the possibility of encountering problems in reproduction. Apart from these, mistakes made by couples who want to have children also affect the sperm count. Using natural products unconsciously and continuing to use them uncontrollably, without knowing the limit, does harm to the person instead of providing benefits. Long-term treatment and drugs used during the treatment also affect the sperm count.
IMSI in Sperm Selection
The IMSI (Intracytoplasmic Morpholgically Selected Sperm Injection) method is the magnification and examination of even the smallest cells using special microscopes. In the past, in the microinjection procedures applied in IVF treatments, living sperm cells could be enlarged up to 400 times. However, with IMSI, sperm can now be enlarged between 6000 and 8000 times. It is possible to examine and detect in detail the disorders called "vacuoles" that indicate the intracellular structures of the sperm and their genetic damage.
The IMSI technique is an extremely useful method. Selected sperm injection; It allows advanced morphological analysis of sperm without damaging the living sperm cell. Sperm with the highest fertilization ability are selected and a high chance of fertilization between 20% and 40% can be obtained with the applied microinjection application.
Precautions to be Considered for Sperm Quality
Couples who want to have children should pay attention to egg and sperm health and be careful in this regard. There are many factors that affect sperm quality, such as those that affect egg quality. One of these factors is undoubtedly a healthy diet. A healthy diet is extremely important for every organ in the body, of course also in sperm health.
In addition, exercise is very important in making you more vigorous and increasing your body's stamina and activity. Regular exercise will make you recover in a short time and will positively affect both your mental and physical health. Alcohol and smoking are substances that affect reproductive cells and should be avoided. This is a proven fact as a result of research. If we gather all these under one title, we can say that one should take care of his own health. Nutrition, avoiding alcohol, smoking, exercising regularly will first affect your health and therefore your sperm quality.
This process, which is sensitive and special for couples who want to have a child, is extremely important for them. In this process, prospective parents experience excitement on the one hand and a lot of stress on the other. Hüma IVF Center, which understands the emotions experienced best and does not spare its support by being by their side throughout the process, is always ready to accompany you.
WHAT IS THE INTRA-LIPID SERUM PROCEDURE AND HOW TO USE IT?
Serum Intralipid Therapy is a treatment used specifically to strengthen the immune system. The immune system plays a big role in the repeated IVF failures and recurrent miscarriages seen in some women. For this purpose, intralipid serum therapy is also used. This is a type of treatment that is started prior to IVF treatment and is applied once a week or every two weeks until the baby's heartbeat is heard, if deemed necessary after pregnancy is achieved. Now let's take a closer look at intralipid serum treatment together ...
What is Intralipid Serum Therapy?
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Intralipid serum therapy is a form of treatment that starts on the day of the transfer and continues throughout the process. Intralipid whey contains fat molecules and soy protein, which strengthens the immune system. The immune system is extremely important for a healthy pregnancy and its continuation. Because half of the child belongs to the father, the mother's body does not reject it. In vitro fertilization failure also occurs in case of rejection, but intralipid therapy can prevent this failure.
For whom and how is intralipid serum treatment applied?
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Intralipid Serum Therapy is an immune-boosting treatment that is commonly used in women with recurrent miscarriage problems and recurrent IVF failures. As a result of studies, it was found that treatment with intralipid serum does not have side effects. Only women can be allergic to some substances in the serum of intralipid, in which case this should be determined in advance. These substances are a number of special ingredients such as glycerin, egg phospholipids. It is preliminarily checked whether the patient is allergic to these substances. If the patient has an allergy, treatment is not applied.
Intralipid whey treatment is carried out with a preparation containing emulsified oil prepared with a special concentration, which takes approximately 45 minutes. Fatty sera are administered to the patient intravenously.
In this article: what is serum intralipid therapy, who is this treatment for, how is intralipid therapy used? We have answered all these questions for you. If you want to get more detailed information about this, we recommend that you visit the website of the HUMA center.
